{"created":"2023-05-15T08:49:53.889461+00:00","id":670,"links":{},"metadata":{"_buckets":{"deposit":"745986bc-24b9-4082-9255-afeb7180fdd9"},"_deposit":{"created_by":3,"id":"670","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"670"},"status":"published"},"_oai":{"id":"oai:jumonji-u.repo.nii.ac.jp:00000670","sets":["7:11:103"]},"author_link":["656","645","640","659","644","641","651","638"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-02-28","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"94","bibliographicPageStart":"83","bibliographicVolumeNumber":"49","bibliographic_titles":[{"bibliographic_title":"十文字学園女子大学紀要"},{"bibliographic_title":"Bulletin of Jumonji University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"グリコーゲンは動物界の貯蔵多糖であり，主に肝臓や骨格筋に存在する．その定量はグリコーゲン合成・分解に関わるメカニズムや調節を理解する上で重要な意味を持つ．従来汎用されてきたフェノール硫酸法やアンスロン硫酸法等は，化学反応に基づく比色法であり，特異性に難点があるとともに，感度が低く，培養細胞スケールの実験では多くの場合，検出限界以下となる．そこで本研究では，グリコーゲンをグルコース単位で分解する酵素であるグルコアミラーゼと蛍光原基質10-Acetyl-3,7-dihydroxyphenoxazineを用いて高感度にグリコーゲンの定量ができるよう酵素蛍光法を検討し，本法の定量可能レンジと検量線の直線性を調べた．その結果，グリコーゲン標準液0.1-20 µg/mLまで良好な直線性が得られた．また，マイクロプレート対応蛍光分光光度計の代用としてリアルタイム定量PCRシステムで定量することも可能であった（定量範囲：0.1-2 µg/mL）．続いて，実際に培養肝細胞HepG2を用い，培養液中のグルコースの有無，グリコーゲン枯渇状態からのグルコース添加による細胞内グリコーゲン量の回復過程を本法により定量した．その結果，培養液中のグルコースの有無による細胞内グリコーゲン量の消長を本法により検出することができた．以上より，本研究の酵素蛍光法は，グリコーゲンを高感度に定量でき，培養細胞スケールのグリコーゲン量の増減を検出することが可能であると結論された．","subitem_description_type":"Abstract"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2424-0591","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"倉若, 美咲樹"},{"creatorName":"クラワカ, ミサキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"638","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐々木, 菜穂"},{"creatorName":"ササキ, ナホ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"651","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"志村, 二三夫"},{"creatorName":"シムラ, フミオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"640","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山崎, 優子"},{"creatorName":"ヤマザキ, ユウコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"641","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KURAWAKA, Misaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"656","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SASAKI, Naho","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"659","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SHIMURA, Fumio","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"644","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"YAMAZAKI, Yuko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"645","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","displaytype":"detail","filename":"紀要49号-08　倉若美咲樹ほか.pdf","filesize":[{"value":"6.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"url":"https://jumonji-u.repo.nii.ac.jp/record/670/files/紀要49号-08　倉若美咲樹ほか.pdf"},"version_id":"2f77c8f1-400c-4e83-a833-653bddd0987a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"培養細胞内グリコーゲンの定量のための酵素蛍光法の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"培養細胞内グリコーゲンの定量のための酵素蛍光法の検討"},{"subitem_title":"A sensitive enzyme fluorimetric assay for intracellular glycogen","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"3","path":["103"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-02-28"},"publish_date":"2019-02-28","publish_status":"0","recid":"670","relation_version_is_last":true,"title":["培養細胞内グリコーゲンの定量のための酵素蛍光法の検討"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T09:03:15.269258+00:00"}